TNF-alpha and metalloproteases as key players in melanoma cells aggressiveness.

ABSTRACT:

Background

Melanoma aggressiveness determines its growth and metastatic potential. This study aimed at identifying new molecular pathways controlling melanoma cell malignancy.

Methods

Ten metastatic melanoma cell lines were characterized by their proliferation, migration and invasion capabilities. The most representative cells were also characterized by spheroid formation assay, gene- and protein- expression profiling as well as cytokines secretion and the most relevant pathways identified through bioinformatic analysis were tested by in silico transcriptomic validation on datasets generated from biopsies specimens of melanoma patients. Further, matrix metalloproteases (MMPs) activity was tested by zymography assays and TNF-alpha role was validated by anti-TNF cell-treatment

SUBMITTER: Rossi S 

PROVIDER: S-EPMC6309098 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature