Scaffold-mediated delivery for non-viral mRNA vaccines.

ABSTRACT: mRNA is increasingly being recognized as a promising alternative to pDNA in gene vaccinations. Only recently, owing to the needs of cancer immunotherapies, has the biomaterials/gene delivery community begun to develop new biomaterial strategies for immunomodulation. Here, we report a novel way to use implantable porous scaffolds as a local gene delivery depot to enhance mRNA vaccine immunization in vitro, and in vivo when compared with conventional bolus injections. We first evaluated transfection efficiencies of single-stranded mRNA condensed and charge neutralized with two lipids (Lipofectamine Messenger MAX^TM LM-MM and Stemfect^TM SF) and two cationic polymers (in vivo-jetPEI™, Poly (β-amino ester)) as gene carriers. As SF demonstrated highest in vitro transfection

SUBMITTER: Chen R 

PROVIDER: S-EPMC6309225 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature