Naringenin Promotes Thermogenic Gene Expression in Human White Adipose Tissue.

ABSTRACT:

Objective

Naringenin, a citrus flavonoid, prevents diet-induced weight gain and improves glucose and lipid metabolism in rodents. There is evidence that naringenin activates brown fat and increases energy expenditure in mice, but little is known about its effects in humans. The goal of this study was to examine the effects of naringenin on energy expenditure in adipose tissue.

Methods

Human white adipocyte cultures (hADSC) and abdominal subcutaneous adipose tissue (pWAT) were treated with naringenin for 7 to 14 days. Expression (quantitative real-time polymerase chain reaction, immunoblotting) of candidate genes involved in thermogenesis and glucose metabolism was measured. Oxygen consumption rate was measured in hADSC using a Seahorse flux analyzer.

Results

In hADSC,

SUBMITTER: Rebello CJ 

PROVIDER: S-EPMC6309263 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature