Using barcoded Zika virus to assess virus population structure in vitro and in Aedes aegypti mosquitoes.

ABSTRACT: Arboviruses such as Zika virus (ZIKV, Flaviviridae; Flavivirus) must replicate in both mammalian and insect hosts possessing strong immune defenses. Accordingly, transmission between and replication within hosts involves genetic bottlenecks, during which viral population size and genetic diversity may be significantly reduced. To help quantify these bottlenecks and their effects, we constructed 4 "barcoded" ZIKV populations that theoretically contain thousands of barcodes each. After identifying the most diverse barcoded virus, we passaged this virus 3 times in 2 mammalian and mosquito cell lines and characterized the population using deep sequencing of the barcoded region of the genome. C6/36 maintain higher barcode diversity, even after 3 passages, than Vero. Additionally, field-caught m

SUBMITTER: Weger-Lucarelli J 

PROVIDER: S-EPMC6309320 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature