In Vitro Assembly of Diverse Bacterial Microcompartment Shell Architectures.

ABSTRACT: Bacterial microcompartments (BMCs) are organelles composed of a selectively permeable protein shell that encapsulates enzymes involved in CO₂ fixation (carboxysomes) or carbon catabolism (metabolosomes). Confinement of sequential reactions by the BMC shell presumably increases the efficiency of the pathway by reducing the crosstalk of metabolites, release of toxic intermediates, and accumulation of inhibitory products. Because BMCs are composed entirely of protein and self-assemble, they are an emerging platform for engineering nanoreactors and molecular scaffolds. However, testing designs for assembly and function through in vivo expression is labor-intensive and has limited the potential of BMCs in bioengineering. Here, we developed a new method for in vitro assembly of define

SUBMITTER: Hagen AR 

PROVIDER: S-EPMC6309364 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature