Next Generation Histology-Directed Imaging Mass Spectrometry Driven by Autofluorescence Microscopy.

ABSTRACT: Histology-directed imaging mass spectrometry (IMS) is a spatially targeted IMS acquisition method informed by expert annotation that provides rapid molecular characterization of select tissue structures. The expert annotations are usually determined on digital whole slide images of histological stains where the staining preparation is incompatible with optimal IMS preparation, necessitating serial sections: one for annotation, one for IMS. Registration is then used to align staining annotations onto the IMS tissue section. Herein, we report a next-generation histology-directed platform implementing IMS-compatible autofluorescence (AF) microscopy taken prior to any staining or IMS. The platform enables two histology-directed workflows, one that improves the registration process between two

SUBMITTER: Patterson NH 

PROVIDER: S-EPMC6309458 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature