An Insight into Glyco-Microheterogeneity of Plasma von Willebrand Factor by Mass Spectrometry.

ABSTRACT: Human plasma von Willebrand Factor (VWF) plays essential roles in primary hemostasis in cooperation with other coagulations factors. There is ample indication that glycosylation affects many biological phases during the protein life cycle. However, comprehensive characterization of all probable N-glycosites simultaneous with O-glycosites is still not fully revealed. Thus, the intention of this exploration was to estimate the occupancy of all canonical N-glycosites besides simultaneous characterization of N- and O-glycoforms. An RP-LC-MS/MS system functionalized with CID and HCD tandem mass was utilized to analyze VWF. N-Glycosite occupancy varied along the protein backbone chain. Out of 257 HCD spectra, 181 characterized glycoforms were specified as either N- or O-glycosites. Sequential cl

SUBMITTER: Gashash EA 

PROVIDER: S-EPMC6309539 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature