MiR-125a restrains cell migration and invasion by targeting STAT3 in gastric cancer cells.

ABSTRACT:

Background

Recently, many microRNAs have been found to be involved in the cancer progression including miR-125a. However, the underlying mechanisms of miR-125a in gastric cancer (GC) remain to be completely elucidated.

Objective

The study was to investigate the functional role of miR-125a and the expression relevance of signal transducer and activator of transcription 3 (STAT3) and hyaluronan synthase 1 (HAS1).

Method

CCK-8 assay, scratch wound healing and transwell assay were conducted to identify the functional role of miR-125a in GC. In addition, using bioinformatics analysis, the target regulation relationship was found in STAT3 and miR-125a. To confirm the relationship, luciferase reporter assay was performed. More importantly, quantitative polymerase chain react

SUBMITTER: Yang L 

PROVIDER: S-EPMC6309784 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature