Distinct Proteomic Signatures in 16 HDL (High-Density Lipoprotein) Subspecies.

ABSTRACT: Objective- HDL (high-density lipoprotein) in plasma is a heterogeneous group of lipoproteins typically containing apo AI as the principal protein. Most HDLs contain additional proteins from a palate of nearly 100 HDL-associated polypeptides. We hypothesized that some of these proteins define distinct and stable apo AI HDL subspecies with unique proteomes that drive function and associations with disease. Approach and Results- We produced 17 plasma pools from 80 normolipidemic human participants (32 men, 48 women; aged 21-66 years). Using immunoaffinity isolation techniques, we isolated apo AI containing species from plasma and then used antibodies to 16 additional HDL protein components to isolate compositional subspecies. We characterized previously described HDL subspecies containing apo

SUBMITTER: Furtado JD 

PROVIDER: S-EPMC6309805 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature