Enteric Virome and Bacterial Microbiota in Children With Ulcerative Colitis and Crohn Disease.

ABSTRACT:

Objectives

We examined the fecal virome and bacterial community composition of children with Crohn disease (CD), ulcerative colitis (UC), and healthy controls to test the hypothesis that unique patterns of viral organisms and/or presence of bacterial pathogens may be identified that could contribute to the pathogenesis of pediatric inflammatory bowel disease (IBD).

Methods

Fecal samples from 24 children (mean 12.2 years) with CD (n = 7) or UC (n = 5) and similar aged controls (n = 12) were processed to determine individual viromes. Viral sequences were identified through translated protein sequence similarity search. Bacterial microbiota were determined by sequencing of the V4 region of the 16S rRNA gene.

Results

Only a few human viruses were detected, so virome analy

SUBMITTER: Fernandes MA 

PROVIDER: S-EPMC6310095 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature