Protein Nanofibril Assemblies Templated by Graphene Oxide Nanosheets Accelerate Early Cell Adhesion and Induce Osteogenic Differentiation of Human Mesenchymal Stem Cells.

ABSTRACT: Bombyx mori silk fibroin (SF) is a promising natural biocompatible protein. However, its interaction with graphene oxide (GO) has never been studied and the resultant SF/GO matrix has not been used to direct stem cell fate. Herein, we found out that mixing SF molecules and GO nanosheets in an aqueous solution can trigger the assembly of SF nanoparticles into oriented nanofibrils due to the guidance of GO nanosheets, forming SF/GO films with unique nanotopographies and improved modulus upon the removal of the solvent. When GO mass percentage in the SF/GO films is 2 and 10%, the SF assemblies are necklace-like nanofibrils (assembled from loosely linked SF nanoparticles) and solid nanofibrils (assembled from densely linked SF nanoparticles) in the resultant films, termed SG2 and SG10, respect

SUBMITTER: Shuai Y 

PROVIDER: S-EPMC6310480 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature