T follicular helper cells restricted by IRF8 contribute to T cell-mediated inflammation.

ABSTRACT: The follicular helper T cell (T_FH) are established regulators of germinal center (GC) B cells, whether T_FH have pathogenic potential independent of B cells is unknown. Based on in vitro T_FH cell differentiation, in vivo T cell transfer animal colitis model, and intestinal tissues of inflammatory bowel disease (IBD) patients, T_FH and its functions in colitis development were analyzed by FACS, ChIP, ChIP-sequencing, WB, ELISA and PCR. Herein we demonstrate that intestinal tissues of patients and colon tissues obtained from Rag1^-/- recipients of naïve CD4⁺ T cells with colitis, each over-express T_FH-associated gene products. Adoptive transfer of naïve Bcl6^-/- CD4⁺ T cells into Rag1^-/-

SUBMITTER: Zhang R 

PROVIDER: S-EPMC6310655 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature