Repression of Divergent Noncoding Transcription by a Sequence-Specific Transcription Factor.

ABSTRACT: Many active eukaryotic gene promoters exhibit divergent noncoding transcription, but the mechanisms restricting expression of these transcripts are not well understood. Here, we demonstrate how a sequence-specific transcription factor represses divergent noncoding transcription at highly expressed genes in yeast. We find that depletion of the transcription factor Rap1 induces noncoding transcription in a large fraction of Rap1-regulated gene promoters. Specifically, Rap1 prevents transcription initiation at cryptic promoters near its binding sites, which is uncoupled from transcription regulation in the protein-coding direction. We further provide evidence that Rap1 acts independently of previously described chromatin-based mechanisms to repress cryptic or divergent transcription. Finally,

SUBMITTER: Wu ACK 

PROVIDER: S-EPMC6310685 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature