Electrostatics, proton sensor, and networks governing the gating transition in GLIC, a proton-gated pentameric ion channel.

ABSTRACT: The pentameric ligand-gated ion channel (pLGIC) from Gloeobacter violaceus (GLIC) has provided insightful structure-function views on the permeation process and the allosteric regulation of the pLGICs family. However, GLIC is activated by pH instead of a neurotransmitter and a clear picture for the gating transition driven by protons is still lacking. We used an electrostatics-based (finite difference Poisson-Boltzmann/Debye-Hückel) method to predict the acidities of all aspartic and glutamic residues in GLIC, both in its active and closed-channel states. Those residues with a predicted pK_a close to the experimental pH₅₀ were individually replaced by alanine and the resulting variant receptors were titrated by ATR/FTIR spectroscopy. E35, located in front of loo

SUBMITTER: Hu H 

PROVIDER: S-EPMC6310827 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature