A Gata4 nuclear GFP transcriptional reporter to study endoderm and cardiac development in the mouse.

ABSTRACT: The GATA zinc-finger transcription factor GATA4 is expressed in a variety of tissues during mouse embryonic development and in adult organs. These include the primitive endoderm of the blastocyst, visceral endoderm of the early post-implantation embryo, as well as lateral plate mesoderm, developing heart, liver, lung and gonads. Here, we generate a novel Gata4 targeted allele used to generate both a Gata4^H2B-GFP transcriptional reporter and a Gata4^FLAG fusion protein to analyse dynamic expression domains. We demonstrate that the Gata4^H2B-GFP transcriptional reporter faithfully recapitulates known sites of Gata4 mRNA expression and correlates with endogenous GATA4 protein levels. This reporter labels nuclei of Gata4

SUBMITTER: Simon CS 

PROVIDER: S-EPMC6310872 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature