Genome-wide analyses supported by RNA-Seq reveal non-canonical splice sites in plant genomes.

ABSTRACT:

Background

Most eukaryotic genes comprise exons and introns thus requiring the precise removal of introns from pre-mRNAs to enable protein biosynthesis. U2 and U12 spliceosomes catalyze this step by recognizing motifs on the transcript in order to remove the introns. A process which is dependent on precise definition of exon-intron borders by splice sites, which are consequently highly conserved across species. Only very few combinations of terminal dinucleotides are frequently observed at intron ends, dominated by the canonical GT-AG splice sites on the DNA level.

Results

Here we investigate the occurrence of diverse combinations of dinucleotides at predicted splice sites. Analyzing 121 plant genome sequences based on their annotation revealed strong splice site conservatio

SUBMITTER: Pucker B 

PROVIDER: S-EPMC6310983 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature