PK-profiling method for identifying the expression of resistance-associated genes in partially resistant oats to crown rust.

ABSTRACT:

Background

Protein kinases play a key role in plant cell homeostasis and the activation of defense mechanisms. Partial resistance to fungi in plants is interesting because of its durability. However, the variable number of minor loci associated with this type of resistance hampers the reliable identification of the full range of genes involved. The present work reports the technique of protein kinase (PK)-profiling for the identification of the PK genes induced in the partially resistant oats line MN841801-1 following exposure to the fungus Puccinia coronata. This is the first time this technique has been used with cDNA (complementary DNA) from a suppression subtractive hybridization library obtained after the hybridization of cDNAs from inoculated and mock-inoculated plants.

Re

SUBMITTER: Loarce Y 

PROVIDER: S-EPMC6311036 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature