Characterization and functional analyses of the human HTR1A gene: 5' regulatory region modulates gene expression in vitro.

ABSTRACT:

Background

The serotonin neurotransmitter (5-HT) and its receptors have important roles in neuropsychiatric disorders such as schizophrenia. The aim of this study was to investigate the functional sequences of the 5' regulation region of the human HTR1A gene to explore the effects on the expression of the 5-HT1A receptor.

Methods

Fourteen recombinant pGL3-basic vectors containing deletion fragments of the HTR1A gene regulatory region were transfected with HEK-293 and SK-N-SH cells. The relative chemiluminescence intensities of different length fragments were analyzed. The JASPAR software was used for the prediction of transcription factors.

Results

In the HEK-293 cells, the relative chemiluminescence intensity of the - 1649 bp to - 1550 bp (ATG + 1) fragment was signi

SUBMITTER: Wu X 

PROVIDER: S-EPMC6311061 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature