Mechanistic aspects of maltotriose-conjugate translocation to the Gram-negative bacteria cytoplasm.

ABSTRACT: Small molecule accumulation in Gram-negative bacteria is a key challenge to discover novel antibiotics, because of their two membranes and efflux pumps expelling toxic molecules. An approach to overcome this challenge is to hijack uptake pathways so that bacterial transporters shuttle the antibiotic to the cytoplasm. Here, we have characterized maltodextrin-fluorophore conjugates that can pass through both the outer and inner membranes mediated by components of the Escherichia coli maltose regulon. Single-channel electrophysiology recording demonstrated that the compounds permeate across the LamB channel leading to accumulation in the periplasm. We have also demonstrated that a maltotriose conjugate distributes into both the periplasm and cytoplasm. In the cytoplasm, the molecule ac

SUBMITTER: Dumont E 

PROVIDER: S-EPMC6311466 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature