Glycolytic Switch Is Required for Transdifferentiation to Endothelial Lineage.

ABSTRACT:

Background

We have previously shown that activation of cell-autonomous innate immune signaling facilitates the transdifferentiation of fibroblasts into induced endothelial cells, and is required to generate induced endothelial cells with high fidelity for endothelial lineage. Recent studies indicate that a glycolytic switch plays a role in induced pluripotent stem cell generation from somatic cells.

Methods

Seahorse and metabolomics flux assays were used to measure the metabolic changes during transdifferentiation in vitro, and Matrigel plug assay was used to assess the effects of glycolysis modulators on transdifferentiation in vivo.

Results

The metabolic switch begins rapidly after activation of innate immunity, before the expression of markers of endothelial lineag

SUBMITTER: Lai L 

PROVIDER: S-EPMC6311718 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature