Total HLA Class I Antigen Loss with the Downregulation of Antigen-Processing Machinery Components in Two Newly Established Sarcomatoid Hepatocellular Carcinoma Cell Lines.

ABSTRACT: Limited information is currently available concerning HLA class I antigen abnormalities in sarcomatoid hepatocellular carcinoma (sHCC). Here, we have analyzed the growth characteristics and HLA class I antigen status of four sHCC cell lines (sHCC29, sHCC63, sHCC74, and SAR-HCV); the first three were newly established in this study. Among the four, sHCC29 showed the highest growth rate in vitro and tumorigenicity in NOD-SCID mice. Unlike sHCC74 and SAR-HCV, both sHCC29 and sHCC63 had no detectable surface HLA class I antigen expression, alongside undetected intracellular β ₂-microglobulin (β ₂m) and marked HLA class I heavy chain and selective antigen-processing machinery (APM) component downregulation. The loss of β ₂m in sHCC29

SUBMITTER: Lei WY 

PROVIDER: S-EPMC6311956 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature