Albumin binding and anticancer effect of magnesium oxide nanoparticles.

ABSTRACT:

Background

Recently, nanomaterials have moved into biological and medicinal implementations like cancer therapy. Therefore, before clinical trials, their binding to plasma proteins like human serum albumin (HSA) and their cytotoxic effects against normal and cancer cell lines should be addressed.

Methods

Herein, the interaction of magnesium oxide nanoparticles (MgO NPs) with HSA was studied by means of fluorescence spectroscopy, circular dichroism (CD) spectroscopy, and docking studies. Afterwards, the cytotoxic impacts of MgO NPs on human leukemia cell line (K562) and peripheral blood mononucleated cells (PBMCs) were evaluated by MTT and flow cytometry assays to quantify reactive oxygen species (ROS) generation and apoptosis.

Results

It was demonstrated that MgO NPs

SUBMITTER: Behzadi E 

PROVIDER: S-EPMC6312066 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature