The influence of subretinal injection pressure on the microstructure of the monkey retina.

ABSTRACT:

Purpose

To investigate the influence of subretinal injection pressure on the microstructure of the retina in a monkey model.

Methods

After vitrectomy, balanced salt solution was injected subretinally into one eye each of four cynomolgus monkeys while controlling the injection pressure. Initially, a pressure of 2 psi was used, and this was gradually increased to determine the minimum required pressure. Subsequent injections were performed at two pressures: minimum (n = 13) and high (n = 6). To compare the influence of these injection pressures on retinal structure, optical coherence tomography (OCT) was performed before surgery and every week afterwards. The monkeys were euthanized and their eyes were enucleated at 1 or 6 weeks after the injections. The eyes were processed fo

SUBMITTER: Takahashi K 

PROVIDER: S-EPMC6312337 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature