Efficient genome editing using CRISPR-Cas-mediated homology directed repair in the ascidian Ciona robusta.

ABSTRACT: Eliminating or silencing a gene's level of activity is one of the classic approaches developmental biologists employ to determine a gene's function. A recently developed method of gene perturbation called CRISPR-Cas, which was derived from a prokaryotic adaptive immune system, has been adapted for use in eukaryotic cells. This technology has been established in several model organisms as a powerful and efficient tool for knocking out or knocking down the function of a gene of interest. It has been recently shown that CRISPR-Cas functions with fidelity and efficiency in Ciona robusta. Here, we show that in C. robusta CRISPR-Cas mediated genomic knock-ins can be efficiently generated. Electroporating a tissue-specific transgene driving Cas9 and a U6-driven gRNA transgene together with a fluo

SUBMITTER: Pickett CJ 

PROVIDER: S-EPMC6312475 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature