BIN1 Induces the Formation of T-Tubules and Adult-Like Ca²⁺ Release Units in Developing Cardiomyocytes.

ABSTRACT: Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) are at the center of new cell-based therapies for cardiac disease, but may also serve as a useful in vitro model for cardiac cell development. An intriguing feature of hESC-CMs is that although they express contractile proteins and have sarcomeres, they do not develop transverse-tubules (T-tubules) with adult-like Ca²⁺ release units (CRUs). We tested the hypothesis that expression of the protein BIN1 in hESC-CMs promotes T-tubules formation, facilitates Ca_V 1.2 channel clustering along the tubules, and results in the development of stable CRUs. Using electrophysiology, [Ca²⁺ ]_i imaging, and super resolution microscopy, we found that BIN1 expression induced T-tubule development in hESC-CM

SUBMITTER: De La Mata A 

PROVIDER: S-EPMC6312737 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature