Characterisation of Streptomyces violascens OC125-8 lipase for oily wastewater treatment.

ABSTRACT: In this study, the lipase-producing bacterium Streptomyces violascens (GenBank number MF621564) was identified, and the extracellular S. violascens OC125-8 lipase produced by this strain was characterised for use in wastewater treatment. The lipase was partially purified by ammonium sulphate precipitation at a final yield of 3.28-fold purification and a recovery of 56%. The S. violascens OC125-8 lipase exhibited optimum catalytic activity at 40 °C and pH 8.0; it was stable at 30-40 °C with more than 86% residual activity after 1 h; it was also stable over a relatively broad pH range of pH 7.0-11.0, retaining 83.3-100% activity. V _max and K _m values were calculated as 0.61 µmol/min/mg and 0.259 mM, respectively. Enzyme activity signif

SUBMITTER: Boran R 

PROVIDER: S-EPMC6312823 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature