MiR-145-targeted HBXIP modulates human breast cancer cell proliferation.

ABSTRACT:

Background

MiR-145 has been identified as a tumor suppressive microRNA in multiple cancers. In this current investigation, we searched for new direct targets of miR-145 and evaluated their effect on breast cancer development.

Methods

Targetscan was used to predict the target genes of miR-145. The targeting of miR-145 on oncogenic HBXIP was verified by luciferase reporter gene analysis. The effect of miR-145 on the level of messenger RNA and protein of HBXIP was evaluated by quantitative real-time PCR and immunoblotting. Correlations between miR-145 and HBXIP, as well as miR-145 expression, were analyzed in 30 paired breast cancer and noncancerous tissues by quantitative real-time PCR. Methyl thiazol tetrazolium and colony formation assays were applied to determine the cell p

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC6312848 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature