The distribution of divinyl chlorophylls a and b and the presence of ferredoxin-NADP⁺ reductase in Prochlorococcus marinus MIT9313 thylakoid membranes.

ABSTRACT: The marine unicellular green cyanobacterium Prochlorococcus marinus MIT9313 belongs to the most abundant and photosynthetically productive genus of cyanobacteria in the oceans. This monophyletic genus use divinyl chlorophyll a (Chl a ₂ ) and b (Chl b ₂ ) to build the photosystems and the membrane-intrinsic Pcb-type antennae. We used the mild detergent n-dodecyl β D-maltopyranoside to solubilize the thylakoid membranes. Gel electrophoresis and sucrose gradient ultracentrifugation was then used to separate the complexes of the photosynthetic apparatus. The proteins and the pigments were identified by mass spectrometry. Protein complexes were characterized biochemically, and the distribution of Chl a _2</

SUBMITTER: Barrera-Rojas J 

PROVIDER: S-EPMC6312871 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature