Long-Term Biogas Production from Glycolate by Diverse and Highly Dynamic Communities.

ABSTRACT: Generating chemical energy carriers and bulk chemicals from solar energy by microbial metabolic capacities is a promising technology. In this long-term study of over 500 days, methane was produced by a microbial community that was fed by the mono-substrate glycolate, which was derived from engineered algae. The microbial community structure was measured on the single cell level using flow cytometry. Abiotic and operational reactor parameters were analyzed in parallel. The R-based tool flowCyBar facilitated visualization of community dynamics and indicated sub-communities involved in glycolate fermentation and methanogenesis. Cell sorting and amplicon sequencing of 16S rRNA and mcrA genes were used to identify the key organisms involved in the anaerobic conversion process. The microb

SUBMITTER: Gunther S 

PROVIDER: S-EPMC6313629 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature