Mapping the Proteome of the Synaptic Cleft through Proximity Labeling Reveals New Cleft Proteins.

ABSTRACT: Synapses are specialized neuronal cell-cell contacts that underlie network communication in the mammalian brain. Across neuronal populations and circuits, a diverse set of synapses is utilized, and they differ in their molecular composition to enable heterogenous connectivity patterns and functions. In addition to pre- and post-synaptic specializations, the synaptic cleft is now understood to be an integral compartment of synapses that contributes to their structural and functional organization. Aiming to map the cleft proteome, this study applied a peroxidase-mediated proximity labeling approach and used the excitatory synaptic cell adhesion protein SynCAM 1 fused to horseradish peroxidase (HRP) as a reporter in cultured cortical neurons. This reporter marked excitatory synapses as measur

SUBMITTER: Cijsouw T 

PROVIDER: S-EPMC6313906 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature