Cardiac mesenchymal cells from diabetic mice are ineffective for cell therapy-mediated myocardial repair.

ABSTRACT: Although cell therapy improves cardiac function after myocardial infarction, highly variable results and limited understanding of the underlying mechanisms preclude its clinical translation. Because many heart failure patients are diabetic, we examined how diabetic conditions affect the characteristics of cardiac mesenchymal cells (CMC) and their ability to promote myocardial repair in mice. To examine how diabetes affects CMC function, we isolated CMCs from non-diabetic C57BL/6J (CMC^WT) or diabetic B6.BKS(D)-Leprdb/J (CMC^db/db) mice. When CMCs were grown in 17.5 mM glucose, CMC^db/db cells showed > twofold higher glycolytic activity and a threefold higher expression of Pfkfb3 compared with CMC^WT cells; however, culture of CMC^db/db cell

SUBMITTER: Mehra P 

PROVIDER: S-EPMC6314032 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature