Hormone-control regions mediate steroid receptor-dependent genome organization.

ABSTRACT: In breast cancer cells, some topologically associating domains (TADs) behave as hormonal gene regulation units, within which gene transcription is coordinately regulated in response to steroid hormones. Here we further describe that responsive TADs contain 20- to 100-kb-long clusters of intermingled estrogen receptor (ESR1) and progesterone receptor (PGR) binding sites, hereafter called hormone-control regions (HCRs). In T47D cells, we identified more than 200 HCRs, which are frequently bound by unliganded ESR1 and PGR. These HCRs establish steady long-distance inter-TAD interactions between them and organize characteristic looping structures with promoters in their TADs even in the absence of hormones in ESR1⁺-PGR⁺ cells. This organization is dependent on the express

SUBMITTER: Le Dily F 

PROVIDER: S-EPMC6314164 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature