Efficient ¹⁵N-¹³C Polarization Transfer by Third-Spin-Assisted Pulsed Cross-Polarization Magic-Angle-Spinning NMR for Protein Structure Determination.

ABSTRACT: We introduce a pulsed third-spin-assisted recoupling experiment that produces high-intensity long-range ¹⁵N-¹³C cross peaks using low radiofrequency (rf) energy. This Proton-Enhanced Rotor-echo Short-Pulse IRradiATION Cross-Polarization (^PERSPIRATIONCP) pulse sequence operates with the same principle as the Proton-Assisted Insensitive-Nuclei Cross-Polarization (^PAINCP) experiment but uses only a fraction of the rf energy by replacing continuous-wave ¹³C and ¹⁵N irradiation with rotor-echo 90° pulses. Using formyl-Met-Leu-Phe (f-MLF) and β1 immunoglobulin binding domain of protein G (GB1) as model proteins, we demonstrate experimentally how ^PERSPIRATIONCP polarization transfer depends on the CP contact time, rf pow

SUBMITTER: Gelenter MD 

PROVIDER: S-EPMC6314680 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature