Sequence-based structural analysis and evaluation of polymorphism in buffalo Nod-like receptor-1 gene.

ABSTRACT: In this study, we have sequence characterized and analyzed the polymorphism in buffalo NOD1 (nucleotide-binding oligomerization domain 1) gene as well as its expression analysis. Full-length sequence analysis of NOD1 revealed this gene in buffalo being conserved with respect to the domain structures, similar to other species. Alternate splice variants having exon3 skipping also identified for the first time in the gene expressed in buffalo-purified peripheral blood mononuclear cells (PBMCs). Phylogenetically ruminant species were found to be clustering together and buffalo displaying maximum similarity with cattle. Sequencing of NOD1 across 12 Indian buffalo breeds identified 23 polymorphic sites within coding region, among which 16 were synonymous and 7 changes found

SUBMITTER: Mishra SK 

PROVIDER: S-EPMC6314939 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature