Human phase I metabolism of the novel synthetic cannabinoid 5F-CUMYL-PEGACLONE.

ABSTRACT:

Purpose

5F-CUMYL-PEGACLONE is a recently emerged γ-carbolinone derived synthetic cannabinoid. The present study aimed to identify phase I metabolites to reliably prove consumption of the substance by urine analysis and to differentiate from the uptake of the non-fluorinated analog CUMYL-PEGACLONE.

Methods

For metabolite characterization, phase I metabolites were analyzed by liquid chromatography-high resolution mass spectrometry after incubation with pooled human liver microsomes. Reliability of the biomarkers was evaluated by analysis of human urine samples (n = 20) by liquid chromatography-triple quadrupole tandem mass spectrometry. Sample preparation included β-glucuronidase treatment followed by liquid-liquid extraction.

Results

In total, 15 m

SUBMITTER: Mogler L 

PROVIDER: S-EPMC6315001 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature