Bio-artificial bone formation model with a radial-flow bioreactor for implant therapy-comparison between two cell culture carriers: porous hydroxyapatite and β-tricalcium phosphate beads.

ABSTRACT: Bone grafting is necessary before dental implant treatment in patients with jaw bone defects. Currently, autologous bone grafting is a major burden on the patient. However, it is impossible to form a sufficient foundation for the implant with a bone-filling agent alone. It is, therefore, necessary to prepare hybrid artificial bone tissue containing osteoblasts and osteoclasts. In this study, mouse MC3T3-E1 pre-osteoblast cells and human embryonic-derived osteoblastic cell line hFOB1.19 were cultured in radial-flow bioreactors (RFB) to form three-dimensional artificial bone filled with porous beads of β-tricalcium phosphate (β-TCP) or hydroxyapatite (HA)-which are clinically used as bone-filling agents-as cell culture carriers. When circulation culturing was performed in the growth medium f

SUBMITTER: Nomoto H 

PROVIDER: S-EPMC6315002 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature