Selective single molecule sequencing and assembly of a human Y chromosome of African origin.

ABSTRACT: Mammalian Y chromosomes are often neglected from genomic analysis. Due to their inherent assembly difficulties, high repeat content, and large ampliconic regions, only a handful of species have their Y chromosome properly characterized. To date, just a single human reference quality Y chromosome, of European ancestry, is available due to a lack of accessible methodology. To facilitate the assembly of such complicated genomic territory, we developed a novel strategy to sequence native, unamplified flow sorted DNA on a MinION nanopore sequencing device. Our approach yields a highly continuous assembly of the first human Y chromosome of African origin. It constitutes a significant improvement over comparable previous methods, increasing continuity by more than 800%. Sequencing native DNA also

SUBMITTER: Kuderna LFK 

PROVIDER: S-EPMC6315018 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature