Electron and Proton Flux for Carbon Dioxide Reduction in Methanosarcina barkeri During Direct Interspecies Electron Transfer.

ABSTRACT: Direct interspecies electron transfer (DIET) is important in diverse methanogenic environments, but how methanogens participate in DIET is poorly understood. Therefore, the transcriptome of Methanosarcina barkeri grown via DIET in co-culture with Geobacter metallireducens was compared with its transcriptome when grown via H₂ interspecies transfer (HIT) with Pelobacter carbinolicus. Notably, transcripts for the F₄₂₀H₂ dehydrogenase, Fpo, and the heterodisulfide reductase, HdrABC, were more abundant during growth on DIET. A model for CO₂ reduction was developed from these results in which electrons delivered to methanophenazine in the cell membrane are transferred to Fpo. The external proton gradient necessary to drive the othe

SUBMITTER: Holmes DE 

PROVIDER: S-EPMC6315138 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature