A Zebrafish Acromegaly Model Elevates DNA Damage and Impairs DNA Repair Pathways.

ABSTRACT: Acromegaly is a pathological condition due to excess growth hormone (GH) secretion. Acromegaly patients exhibit a deterioration of health and many associated complications, such as cardiovascular issues, arthritis, kidney diseases, muscular weakness, and colon cancer. Since these complications are generalized throughout the body, we investigated the effect of GH excess on cellular integrity. Here, we established stable acromegaly model zebrafish lines that overexpress tilapia GH and the red fluorescence protein (RFP) reporter gene for tracking GH gene expression throughout generations, and performed RNA-Seq data analysis from different organs. Intriguingly, heatmap and Expression2Kinases (X2K) analysis revealed the enrichment of DNA damage markers in various organs. Moreover, H2A.X immunos

SUBMITTER: Elbialy A 

PROVIDER: S-EPMC6315448 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature