Fucoidan Sulfatases from Marine Bacterium Wenyingzhuangia fucanilytica CZ1127^T.

ABSTRACT: Fucoidans belong to a structurally heterogeneous class of sulfated polysaccharides isolated from brown algae. They have a wide spectrum of biological activities. The complex structures of these polysaccharides hinder structure-activity relationships determination. Fucoidan sulfatases can make useful tools for the determination of the fine chemical structure of fucoidans. In this study, identification and preparation of two recombinant sulfatases able to catalyze the cleavage of sulfate groups from fragments of fucoidan molecules is described for the first time. Two genes of sulfatases swf1 and swf4 of the marine bacterium Wenyingzhuangia fucanilytica CZ1127^T were cloned and the proteins were produced in Escherichia coli cells. Sulfatases SWF1 and SWF4

SUBMITTER: Silchenko AS 

PROVIDER: S-EPMC6315715 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature