Gas Chromatography Mass Spectrometry (GC-MS) Quantification of Metabolites in Stool Using ¹³C Labelled Compounds.

ABSTRACT: It has become increasingly important to qualitatively and quantitatively assess the volatile metabolites in a range of bodily fluids for use in monitoring health. There has been relatively little work on the quantitative analysis of compounds, particularly with respect to the effects of ethnicity or geographic location. A novel method for the quantification of compounds in stool using ¹³C labelled compounds as internal standards is presented. Using thermal desorption gas chromatography mass spectrometry, stool samples from 38 healthy volunteers were analysed. The ¹³C labelled compounds, acetone, ethyl butanoate, ethanoic acid, butanoic acid, 3-methylbutanoic acid, and indole, were added as internal standards. This process mimics the solubility characteristics of the c

SUBMITTER: Gould O 

PROVIDER: S-EPMC6316270 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature