A Systematic Study on DNA Barcoding of Medicinally Important Genus Epimedium L. (Berberidaceae).

ABSTRACT: Genus Epimedium consists of approximately 50 species in China, and more than half of them possess medicinal properties. The high similarity of species' morphological characteristics complicates the identification accuracy, leading to potential risks in herbal efficacy and medical safety. In this study, we tested the applicability of four single loci, namely, rbcL, psbA-trnH, internal transcribed spacer (ITS), and ITS2, and their combinations as DNA barcodes to identify 37 Epimedium species on the basis of the analyses, including the success rates of PCR amplifications and sequencing, specific genetic divergence, distance-based method, and character-based method. Among them, character-based method showed the best applicability for identifying Epimedium<

SUBMITTER: Guo M 

PROVIDER: S-EPMC6316794 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature