Cloning and Functional Verification of Genes Related to 2-Phenylethanol Biosynthesis in Rosa rugosa.

ABSTRACT: In China, Rosa rugosa is cultivated as a source of natural perfumes. Rose essential oil is known as "liquid gold", given its high economic and health value. 2-phenylethanol accounts for more than 10% of the total mass fraction of the essential oil derived from R. rugosa. The regulatory mechanisms underlying 2-phenylethanol metabolism in R. rugosa, however, remain unclear. In this study, RrAAAT and RrPPDC1, two genes related to 2-phenylethanol synthesis, were cloned from R. rugosa. Expression analysis revealed that RrAAAT and RrPPDC1 were highly expressed in rose flowers in the full opening and withering stages, and in calyxes. The overexpression vectors of RrAADC, RrAAAT, and RrPPDC1 were established and transform

SUBMITTER: Sheng L 

PROVIDER: S-EPMC6316838 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature