Cancer immunophenotyping by seven-colour multispectral imaging without tyramide signal amplification.

ABSTRACT: Checkpoint blockade immunotherapies have revolutionised cancer treatment in the last decade. Nevertheless, these are only beneficial for a small proportion of cancer patients. Important prognosticators for response to immunotherapy are the mutation burden of tumours as well as the quality and quantity of tumour-infiltrating immune cells. High-throughput multiplex immunophenotyping technologies have a central role in deciphering the complexity of anti-tumour immune responses. Current techniques for the immunophenotyping of solid tumours are held back by the lack of spatial context, limitations in the number of targets that can be visualised simultaneously, and/or cumbersome protocols. We developed a tyramide signal amplification-free method for the simultaneous detection of seven cellular t

SUBMITTER: Ijsselsteijn ME 

PROVIDER: S-EPMC6317065 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature