Functional dissection of the developmentally restricted BEN domain chromatin boundary factor Insensitive.

ABSTRACT:

Background

Boundaries in the Drosophila bithorax complex delimit autonomous regulatory domains that activate the parasegment (PS)-specific expression of homeotic genes. The Fab-7 boundary separates the iab-6 and iab-7 regulatory domains that control Abd-B expression in PS11 and PS12. This boundary is composed of multiple functionally redundant elements and has two key activities: it blocks crosstalk between iab-6 and iab-7 and facilitates boundary bypass.

Results

Here, we have used a structure-function approach to elucidate the biochemical properties and the in vivo activities of a conserved BEN domain protein, Insensitive, that is associated with Fab-7. Our biochemical studies indicate that in addition to the C-terminal BEN DNA-binding domain, Insv has two domains that medi

SUBMITTER: Fedotova A 

PROVIDER: S-EPMC6317261 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature