Induction of functional islet-like cells from human iPS cells by suspension culture.

ABSTRACT:

Introduction

To complement islet transplantation for type1 diabetic patients, cell-based therapy using pluripotent stem cells such as ES cells and iPS cells is promising. Many papers have already reported the induction of pancreatic β cells from these cell types, but a suspension culture system has not usually been employed. The aim of this study is to establish a suspension culture method for inducing functional islet-like cells from human iPS cells.

Methods

We used 30 ml spinner type culture vessels for human iPS cells throughout the differentiation process. Differentiated cells were analyzed by immunostaining and C-peptide secretion. Cell transplantation experiments were performed with STZ-induced diabetic NOD/SCID mice. Blood human C-peptide and glucagon levels were meas

SUBMITTER: Yabe SG 

PROVIDER: S-EPMC6317273 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature