Single-cell RNA-seq analysis reveals the progression of human osteoarthritis.

ABSTRACT:

Objectives

Understanding the molecular mechanisms underlying human cartilage degeneration and regeneration is helpful for improving therapeutic strategies for treating osteoarthritis (OA). Here, we report the molecular programmes and lineage progression patterns controlling human OA pathogenesis using single-cell RNA sequencing (scRNA-seq).

Methods

We performed unbiased transcriptome-wide scRNA-seq analysis, computational analysis and histological assays on 1464 chondrocytes from 10 patients with OA undergoing knee arthroplasty surgery. We investigated the relationship between transcriptional programmes of the OA landscape and clinical outcome using severity index and correspondence analysis.

Results

We identified seven molecularly defined populations of chondrocytes

SUBMITTER: Ji Q 

PROVIDER: S-EPMC6317448 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature