Single-particle reconstruction statistics: a diagnostic tool in solving biomolecular structures by cryo-EM.

ABSTRACT: In single-particle analysis (SPA), the aim is to obtain a 3D reconstruction of a biological molecule from 2D electron micrographs to the highest level of detail or resolution as possible. Current practice is to collect large volumes of data, hoping to reach high-resolution maps through sheer numbers. However, adding more particles from a specific data set eventually leads to diminishing improvements in resolution. Understanding what these resolution limits are and how to deal with them are important in optimization and automation of SPA. This study revisits the theory of 3D reconstruction and demonstrates how the associated statistics can provide a diagnostic tool to improve SPA. Small numbers of images already give sufficient information on micrograph quality and the amount of data requir

SUBMITTER: Heymann JB 

PROVIDER: S-EPMC6317460 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature