IRF1 inhibits the proliferation and metastasis of colorectal cancer by suppressing the RAS-RAC1 pathway.

ABSTRACT:

Background

Interferon regulatory factor 1 (IRF1) plays a role in the immune response, cellular necrosis, DNA damage, and DNA repair, offering an attractive target for anticancer treatment. However, little is known about the role of IRF1 in the regulation of CRC progression.

Methods

Quantitative reverse transcription-PCR, Western blot, and immunohistochemistry were used to examine the expression level of IRF1; Cell Counting Kit-8, migration assay, and xenograft mouse models were used to examine the function of IRF1 in CRC cell lines; a ChIP assay was used to examine the binding between IRF1 and Ras association domain-containing protein 5 (RASSF5).

Results

IRF1 expression was lower in colorectal cancer (CRC) than in normal mucosa and the IRF1 expression level was invers

SUBMITTER: Hong M 

PROVIDER: S-EPMC6317468 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature