DNA Methyltransferase 1 Controls Nephron Progenitor Cell Renewal and Differentiation.

ABSTRACT:

Background

Nephron number is a major determinant of long-term renal function and cardiovascular risk. Observational studies suggest that maternal nutritional and metabolic factors during gestation contribute to the high variability of nephron endowment. However, the underlying molecular mechanisms have been unclear.

Methods

We used mouse models, including DNA methyltransferase (Dnmt1, Dnmt3a, and Dnmt3b) knockout mice, optical projection tomography, three-dimensional reconstructions of the nephrogenic niche, and transcriptome and DNA methylation analysis to characterize the role of DNA methylation for kidney development.

Results

We demonstrate that DNA hypomethylation is a key feature of nutritional kidney growth restriction in vitro and in viv

SUBMITTER: Wanner N 

PROVIDER: S-EPMC6317605 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature